Nature11 个月
Detecting protein-protein interactions with GFP-fragment reassembly
To purify the amplification products, extract each reaction once with phenol/chloroform/isoamyl alcohol (25: 24:1, v/v/v) and then with chloroform/isoamyl alcohol (29:1, v/v) and recover the DNA ...
Nature1 个月
Purification of RNA from cells and tissues by acid phenol–guanidinium thiocyanate ...
2 | Transfer the homogenate to a fresh tube and sequentially add 0.1 ml of 2 M sodium acetate (pH 4.0), 1 ml of phenol and 0.2 ml of 49:1 (wt/vol) chloroform/isoamyl alcohol per milliliter of ...