In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.
A miniature head-mounted two-photon microscope small enough ... All the microscope optics reside in the miniature microscope, while the excitation and emission light are delivered and collected ...
Nobel Prize in Chemistry was awarded for the development of super-resolved fluorescence microscopy, including STED ...