ARH Is a Modular Adaptor Protein That Interacts with the LDL …
In this report, we examined whether ARH interacts with the LDLR tail and with the two major structural components of clathrin-coated pits, clathrin and AP-2. Our data indicate that evolutionarily conserved motifs in ARH mediate direct interactions between ARH and the LDLR, ARH and the terminal domain of the clathrin heavy chain, and ARH and the ...
ARH is a modular adaptor protein that interacts with the LDL …
2002年11月15日 · Mutations in the phosphotyrosine binding domain protein ARH cause autosomal recessive hypercholesterolemia, a disorder caused by defective internalization of low density lipoprotein receptors (LDLR) in the liver. To examine the function of ARH, we used pull-down experiments to test for interactions …
The clathrin adaptor proteins ARH, Dab2, and numb play
2014年11月28日 · The uptake of circulating low density lipoproteins (LDL) is mediated by LDL receptor (LDLR) through clathrin-dependent endocytosis. At the early stage of this process, adaptor proteins ARH and Dab2 specifically bind the endocytic signal motif in LDLR and recruit clathrin/AP2 to initiate internalizat …
The Clathrin Adaptor Proteins ARH, Dab2, and Numb Play Distinct …
The uptake of circulating low density lipoproteins (LDL) is mediated by LDL receptor (LDLR) through clathrin-dependent endocytosis. At the early stage of this process, adaptor proteins ARH and Dab2 specifically bind the endocytic signal motif in LDLR and recruit clathrin/AP2 to …
The autosomal recessive hypercholesterolemia (ARH) protein
2002年11月25日 · PTB domains bind to the consensus sequence FXNPXY, corresponding to the internalization motif of the LDL receptor. We show here that in addition to the FXNPXY sequence, ARH binds directly to soluble clathrin trimers and to clathrin adaptors by a mode involving the independently folded appendage domain of the β subunit.
Genetics, Clinical Phenotype, and Molecular Cell Biology of …
2003年9月4日 · Structural features of the ARH protein and its capacity to interact simultaneously with the internalization sequence of the LDL receptor, plasma membrane phospholipids, and the clathrin endocytic machinery suggest how ARH can play a pivotal role in gathering the LDL receptor into forming endocytic carrier vesicles.
The Modular Adaptor Protein Autosomal Recessive ... - ScienceDirect
2005年12月9日 · The results indicate that the PTB domain, the clathrin box, and the AP-2 binding motif of ARH interact in a combinatorial fashion to promote the localization of LDLR to clathrin-coated pits and the clearance of circulating LDL by the liver.
(PDF) ARH Is a Modular Adaptor Protein That Interacts with the …
2002年12月1日 · To examine the function of ARH, we used pull-down experiments to test for interactions between ARH, the LDLR, and proteins involved in clathrin-mediated endocytosis. The phosphotyrosine binding...
ARH cooperates with AP-1B in the exocytosis of LDLR in polarized ...
2011年4月4日 · We propose that during biosynthetic delivery or endocytic recycling, the FxNPxY sorting determinant of LDLR-CT27 is recognized by ARH, which subsequently bridges LDLR-CT27 to AP-1B by interacting with AP-1B’s β1 subunit, thereby facilitating incorporation of LDLR-CT27 into nascent clathrin-coated vesicles (Fig. 5 B).
The modular adaptor protein autosomal recessive ... - PubMed
2005年12月9日 · These results demonstrate that ARH must bind the LDLR tail and either clathrin or AP-2 to promote receptor clustering and internalization of LDL. Autosomal recessive hypercholesterolemia is characterized by a cell type-specific defect in low density lipoprotein receptor (LDLR) endocytosis.