Directed cardiomyocyte differentiation from human pluripotent stem ...
2012年12月20日 · The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to functional cardiomyocytes in a completely defined, growth factor– and serum-free system by temporal ...
A method for differentiating human induced pluripotent stem cells ...
2020年10月28日 · We adapted protocols for differentiating human pluripotent stem cells to functional human cardiomyocytes in a 96-well microplate format. The resulting cardiomyocytes expressed cardiac specific...
Induced pluripotent stem cell-derived cardiomyocyte in vitro …
2024年11月8日 · Recent innovations in differentiating cardiomyocytes from human induced pluripotent stem cells (hiPSCs) have unlocked a viable path to creating in vitro cardiac models. Currently, hiPSC-derived...
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells ...
Human cardiomyocytes derived from pluripotent stem cells (PSCs) are the only viable source for new cardiomyocytes currently available. The reason is that adult cardiomyocytes are non-proliferative, and adult stem cells are difficult to differentiate into cardiomyocytes.
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells …
2020年6月19日 · In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014, Lian et al., 2012, Lin et al., 2017).
Robust cardiomyocyte differentiation from human pluripotent stem cells …
2012年5月29日 · Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation ...
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells …
2020年6月19日 · To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation. For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).
Directed cardiomyocyte differentiation from human pluripotent stem ...
Directed differentiation of specific lineages from human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), is the first critical step toward constructing development or disease models, drug screening tools, or cellular therapies from hPSCs.
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells …
In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014, Lian et al., ...
Efficient differentiation of cardiomyocytes from human pluripotent stem ...
Efficient cardiomyocyte differentiation, a prerequisite for the application of stem cell-derived cardiomyocytes, can be achieved with a growth factor-guided method. Undifferentiated cells are sequentially treated with activin A and BMP4 in a serum-free and insulin-free medium and then maintained in a serum-free medium with insulin.