Gene Cloning using Plasmids: Molecular Cloning Intro - SciGine
Gene Cloning using Plasmids: Molecular Cloning Intro - SciGine
Gene cloning using plasmids and PCR allows you to modify cellular DNA. This power molecular cloning technique is explained step-by-step in this blog post.
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Label the image to review the process of gene cloning.SplicingandLigationRecombinantPlasmidPlasmidTranslationTranscriptionIsolated GeneGenetic DonorProtein ProductResetCloning starts with two main ingredients: the gene you are interested in (in this case, interferon, which has been cut out of its genome using appropriate restriction enzymes, and a cloning vector, which is usually a plasmid. Many different types of plasmids are available commercially.The first step in cloning is to prepare the is
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